The FKM (Fluorescence Kinetic Microscope) multispectral fluorescence dynamic microscopy imaging system is currently the most powerful and comprehensive instrument for studying plant microfluorescence. It is a customized microscopy imaging system based on FluorCam chlorophyll fluorescence imaging technology. It consists of an enhanced microscope with expandable components, a high-resolution CCD camera, an excitation light source group, a spectrometer, a temperature control module, corresponding control units, and dedicated workstations and analysis software. It can not only perform Fv/Fm, Kautsky induction effect, fluorescence quenching, OJIP rapid fluorescence response curve, QA re oxidation and other chlorophyll fluorescence and MCF multispectral fluorescence imaging analysis on microalgae, single cells, single chloroplasts and even granule matrix thylakoid fragments; It is also possible to measure any fluorescence excitation and fluorescence release band by exciting the light source group, thereby performing imaging analysis of fluorescent proteins such as GFP, DAPI, DiBAC4, SYTOX, CTC, fluorescent dyes, as well as algal specific fluorescent pigments such as phycocyanin, phycoerythrin, and phycobilin; It is also possible to use a spectrometer for spectral analysis of various fluorescent substances, distinguish between different chromophores (such as PSI and PSII, and various light harvesting pigment complexes), and conduct in-depth analysis.

The FKM multispectral fluorescence dynamic microscopy imaging system has truly made fluorescence imaging technology a probe for studying the mechanism of photosynthesis, enabling researchers to deeply understand the process of photosynthesis and the various changes that occur during it at the cellular and subcellular levels in algae and higher plants. It provides the most powerful tool for directly studying the working mechanism of the photosynthetic system in chloroplasts. FKM, as a dual tool for the microscopic study of algal/plant phenotypes and genotypes, has been widely recognized by the academic community and has achieved a large number of scientific research results.

Functional Features

•Built in programs for current chlorophyll fluorescence research, such as Fv/Fm, Kautsky induction effect, fluorescence quenching, OJIP rapid fluorescence response curve, QA re oxidation, etc., can obtain more than 70 parameters.

•Equipped with 10x, 20x, 40x, 63x, and 100x specialized bioluminescence objectives, chloroplasts and their emitted fluorescence can be clearly observed.

•The excitation light source group includes infrared light, red light, blue light, green light, white light, ultraviolet light, and far red light. By using the three colors of red, blue, and green, any color in the visible spectrum can be extracted, and any pigment molecule or chromophore in plants/algae can be studied.

•Can perform imaging analysis of fluorescent proteins and dyes such as GFP, DAPI, DiBAC4, SYTOX, CTC, etc

•High resolution spectrometers can deeply analyze various fluorescent spectra.

•The temperature control system can ensure that the experimental samples are measured under the same temperature conditions, improve experimental accuracy, and can also be used for high/low temperature stress research.

application area

•Microscopic Structure of Single Cells, Single Chloroplasts, and Granulocyte Matrix Cystoid Fragments in Microalgae, Large Algae/Higher Plants: A Study of Plant Photosynthetic Physiology

•Research on algae/plant stress

•Research on biotic and abiotic stress

•Research on the Stress Resistance and Susceptibility of Algae/Plants

•Mutant screening and photosynthetic mechanism research

•Assessment of Algae Growth and Yield

•The relationship between algal specific pigments and photosynthesis

•Research on the Interaction between Algae/Plants and Microorganisms

•Research on the Interaction between Algae/Plants and Protozoa

•Genetic Engineering and Molecular Biology Research

Measure the sample

•Live plant slices

•Plant epidermis

•plant cell

•Various single-cell and multicellular microalgae such as green algae, blue-green algae, etc

•Chloroplast extract

•Thylakoid extract

•Protozoa containing chloroplasts

Working principle

During the FKM analysis process, dynamic fluorescence of various chromophores in plant samples is excited by a series of filters and spectroscopes embedded in a 6-position filtering wheel and an excitation light source group connected to the microscope. The fluorescence excited by the sample is magnified under a microscope for fluorescence spectroscopy analysis and fluorescence kinetic imaging analysis. The SM 9000 spectrometer is connected to a microscope through optical fibers for excitation fluorescence spectroscopy analysis. The high-resolution CCD camera installed on top of the microscope is used for fluorescence dynamics imaging analysis. The entire work process is automatically carried out through workstations and control units according to pre-set programs. During the measurement process, the temperature of experimental samples such as algae and plant cells can be regulated through a temperature control module. Peristaltic pumps can achieve continuous measurement of algae cultivation.

Instrument composition

1.Enhanced microscope

2.High resolution CCD camera

3.Excitation light source group

4.SM 9000 spectrometer

5.Main control unit

6.Workstation and software

7.Control unit of temperature control module

8.6-digit filtering wheel